Blood Cells Separation and Sorting Techniques of Passive Microfluidic Devices: From Fabrication to Applications

Continuous separation of blood cells in spiral microfluidic devices.

Blood cell sorting is critical to sample preparation for both clinical diagnosis and therapeutic research. The spiral inertial microfluidic devices can achieve label-free, continuous separation of cell mixtures with high throughput and efficiency. The devices utilize hydrodynamic forces acting on cells within laminar flow, coupled with rotational Dean drag due to curvilinear microchannel geomet...

Microfluidic Devices Fabrication for Bioelectrokinetic System Applications

Nonlithographic fabrication of microfluidic devices.

A facile nonlithographic method for expedient fabrication of microfluidic devices of poly(dimethylsiloxane) is described. Positive-relief masters for the molds are directly printed on smooth substrates. For the formation of connecting channels and chambers inside the polymer components of the microfluidic devices, cavity-forming elements are adhered to the surfaces of the masters. Using this no...

Toner and paper-based fabrication techniques for microfluidic applications.

The interest in low-cost microfluidic platforms as well as emerging microfabrication techniques has increased considerably over the last years. Toner- and paper-based techniques have appeared as two of the most promising platforms for the production of disposable devices for on-chip applications. This review focuses on recent advances in the fabrication techniques and in the analytical/bioanaly...

From microdroplets to microfluidics: selective emulsion separation in microfluidic devices.

Microdroplets show great promise as a new high-throughput technology in chemistry, biochemistry, and molecular biology. Microdroplets can be generated at rates in excess of several thousands per second and accurately formulated using minute amounts of small molecules, DNA, proteins, or cells. Furthermore, integrated active elements can be used to control individual droplets. With the technology...